LightCycler® Probe Design Software 2.0 from Roche

Product Specs
ItemLightCycler^® Probe Design Software 2.0
CompanyRoche
Price Supplier Page
Catalog Number04342054001

Specifications/Features

Platform:
Windows 2000, Windows XP

Functions:
The LightCycler Probe Design Software 2.0 is optimized to design:

Primer / probe sets for quantitative PCR
Primer / probe sets for for detection of mutations using Melting Curve Analysis with the LightCycler Instruments.
Primers only for PCR using SYBR Green I detection
SimpleProbe probes for genotyping with the LightCycler Instrument.
Up to 4 different HybProbe pairs for multiplex quantitative PCR and mutation detection with the LightCycler 2.0 Instrument.

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Spatial scale influences the distribution of viral diversity in the eukaryotic virome of the mosquito Culex pipiensVirus EvolutionSeptember 16, 2023Patricia Gil, Antoni Exbrayat, Etienne Loire, Ignace Rakotoarivony, Florian Charriat, Côme Morel, Thierry Baldet, Michel Boisseau, Albane Marie, Benoît Frances, et al.clusters. At least three primer pairs were designed and tested per vOTU. Primer design was done with LightCycler Probe design Software 2.0 (Roche) and based on the contigs of each vOTU. Primer pairs were used in RT-qPCR reactions using mosquito

(2) Genomic Landscape of Primary Resistance to Osimertinib Among Hispanic Patients with EGFR-Mutant Non-Small Cell Lung Cancer (NSCLC): Results of an Observational Longitudinal Cohort StudyTargeted OncologyMay 1, 2023Diego F. Chamorro, Andrés F. Cardona, July Rodríguez, Alejandro Ruiz-Patiño, Oscar Arrieta, Darwin A. Moreno-Pérez, Leonardo Rojas, Zyanya Lucia Zatarain-Barrón, Dora V. Ardila, Lucia Viola, et al.reaction with specific primers and probes for each gene. The primer and probe sets were designed using the LightCycler® Probe Design Software 2.0 (Roche Diagnostics Corporation, Indianapolis, Indiana) according to their Ref Seq (National Center for

(3) Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269IWorld Journal of GastroenterologyMarch 21, 2023Kijeong Kim, Yu-Min Choi, Dong Hyun Kim, Junghwa Jang, Won Hyeok Choe, Bum-Joon KimHBV WT and variant DNA in clinical samples. Primer and LNA probe design Primers were designed using LightCycler Probe Design Software 2.0 (LC PDS 2.0) Version 1.0.R.36 (Roche). The primers were designed to have high melting temperatures (>

(4) Validation of Multiplex PCR and Serology Detecting Helicobacter Species in MiceMicroorganismsJanuary 18, 2023Julia Butt, Mareike Schmitz, Bernhard Berkus, Katja Schmidt, Daniela Höflergyrase A gene and gamma-glutamyltransferase if present. Primers and probes were designed using the LightCycler Probe Design Software 2.0 (Roche). To ensure high clinical specificity, forward or backward primers or probes were designed with

(5) Maternal dietary methionine restriction alters the expression of energy metabolism genes in the duckling liverBMC GenomicsDecember 1, 2022Aurélie Sécula, Hervé Chapuis, Anne Collin, Lisa E. Bluy, Agnès Bonnet, Loys Bodin, Laure Gress, Alexis Cornuez, Xavier Martin, Cécile M. D. Bonnefont, et al.either side of an intron, and for an annealing temperature of 60 °C, using either Primer3Plus [47] or LightCycler® Probe Design Software 2.0 (Roche Applied Science). The primer sequences were blasted to the databases to confirm that they were

(6) Screening of organoids derived from patients with breast cancer implicates the repressor NCOR2 in cytotoxic stress response and antitumor immunityNature CancerMay 26, 2022Kelvin K. Tsai, Shenq-Shyang Huang, Jason J. Northey, Wen-Ying Liao, Chung-Chi Hsu, Li-Hsin Cheng, Michael E. Werner, Chih-Pin Chuu, Chandrima Chatterjee, Jonathon N. Lakins, et al.LightCycler FastStart DNA MASTERPLUS SYBR Green I Kit and the LightCycler System (Roche). Oligonucleotide primers were designed using LightCycler Probe Design Software 2.0 (Roche) or Primer Bank (http://pga.mgh.harvard.edu/primerbank/index.html) (Supplementary Table 1). Transcript

(7) Utilization of Plant Architecture Genes in Soybean to Positively Impact Adaptation to High Yield EnvironmentsFrontiers in Plant ScienceMay 24, 2022Jeong-Hwa Kim, Andrew Scaboo, Vincent Pantalone, Zenglu Li, Kristin BilyeuCATAGGATACTAACCAACG-3′ at 0.2-μM final concentration). The SimpleProbes were designed using Roche Applied Science LightCycler Probe Design software 2.0 (version 1.0, February 2004), and the probes were ordered from Flourescentric, Inc. (Park City, UT). Reactions

(8) Pharmacokinetic Assessment of Staphylococcal Phage K Following Parenteral and Intra-articular Administration in RabbitsFrontiers in PharmacologyMay 20, 2022Katherine M.C. Totten, Scott A. Cunningham, Naomi M. Gades, Athema Etzioni, Robin Patelphage K DNA polymerase I gene, polA (Genbank Accession KF766114, Bethesda, MD), were designed with LightCycler™ Probe Design Software 2.0 (Roche Molecular Systems, Pleasanton, CA) (Supplementary Table S1). qPCR master mix included 0.25 μM

(9) DRD2 Taq1A Polymorphism-Related Brain Volume Changes in Parkinsonâ€™s Disease: Voxel-Based MorphometryParkinson's DiseaseMarch 28, 2022Kenji Ohira, Hajime Yokota, Shigeki Hirano, Motoi Nishimura, Hiroki Mukai, Takuro Horikoshi, Setsu Sawai, Yoshitaka Yamanaka, Tatsuya Yamamoto, Shingo Kakeda, et al.sequencing regions using High-Resolution Melting (HRM) analysis. Primers for HRM analysis were made using LightCycler Probe Design Software 2.0 (Roche), and the HRM polymerase chain reaction (HRM-PCR) was performed using a LightCycler 480 Instrument

(10) Mitochondrial Haplogroup Classification of Ancient DNA Samples Using HaplotrackerBioMed Research InternationalMarch 18, 2022Kijeong Kim, Dong-han Kim, Kyung-yong Kimfor the nested HRM real-time PCR to detect variations. All the primer sequences were designed using LightCycler® Probe Design Software 2.0 (LC PDS 2.0) Version 1.0.R.36 (Roche). The software setting for reaction conditions was as follows:

(11) Skeletal Ryanodine Receptors Are Involved in Impaired Myogenic Differentiation in Duchenne Muscular Dystrophy PatientsInternational Journal of Molecular SciencesNovember 30, 2021Pierre Meyer et al.system (Roche Diagnostics, Meylan, France). PCR primers (Supplementary Table S1) were designed using the LightCycler Probe Design software 2.0. The expression levels were determined with the LightCycler analysis software (Release 1.5.0) relative

(12) Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stagesSTAR ProtocolsSeptember 17, 2021Masafumi Tsuboi, Yukiko Gotohalgorithms,Software and algorithms,Software and algorithms ,, LightCycler Probe Design Software 2.0,Roche,Cat#04342054001 "LightCycler 480 Software, version 1.5.1",Roche,https://lifescience.roche.com/global_en/products/lightcycler14301-480-software-version-15.html ,, Other,Other,Other ,, 100-mm

(13) Rapid screening for mutations associated with malignant hyperthermia using high-resolution melting curve analysisTzu Chi Medical JournalApril 26, 2021Hsien-Tse Huang, Yuan-Ji Wang, Chun-Yu Chen, Hsien-Tse Huang, Chun-Yu Chenanalyzed at the Tzu Chi Medical Center, Hualien. Primers The RYR1 exon primers were designed using LightCycler Probe Design Software 2.0 (Roche) [Table 1]. To ensure adequately short amplicons for HRM, the maximum length was set to 250 bp

(14) Phenotypic switch of smooth muscle cells in paediatric chronic intestinal pseudo‐obstruction syndromeJournal of Cellular and Molecular MedicineApril 1, 2021Delphine Martire et al.using the LightCycler technology (Roche Diagnostics). PCR primers (Table S1) were designed using the LightCycler Probe Design software‐2.0. Each sample was analysed in three independent experiments done in triplicate. Expression levels were

(15) The Genome Segments of Bluetongue Virus Differ in Copy Number in a Host-Specific MannerJournal of VirologyDecember 9, 2020Yannis Moreau, Patricia Gil, Antoni Exbrayat, Ignace Rakotoarivony, Emmanuel Bréard, Corinne Sailleau, Cyril Viarouge, Stephan Zientara, Giovanni Savini, Maria Goffredo, et al.instructions. Development and validation of the quantitative PCR designs. Primers were designed with the LightCycler probe design software 2.0 (Roche) using as the template the segment sequences of strain BTV4‐16‐03 (GenBank accession numbers

(16) Monoamine oxidase B rs1799836 G allele polymorphism is a risk factor for early development of levodopa-induced dyskinesia in Parkinson's diseaseeNeurologicalSciJune 1, 2020Shoko Kakinuma, Minako Beppu, Setsu Sawai, Akitoshi Nakayama, Shigeki Hirano, Yoshitaka Yamanaka, Tatsuya Yamamoto, Chigusa Masafumi, Xiamuxiya Aisihaer, Alimasi Aersilan, et al.[17], [18], [19], [20], [21]] (Supplementary Table 1). 2.4 HRM analysis Primers were designed by LightCycler Probe Design Software 2.0 (Roche Diagnostics). HRM polymerase chain reaction (HRM-PCR) was performed on a LightCycler 480 Instrument

(17) Optimisation of cell and ex vivo culture conditions to study vascular calcificationPLOS ONEMarch 6, 2020Nathalie Gayrard, Karen Muyor, Cécile Notarnicola, Flore Duranton, Bernard Jover, Àngel Argilésdye chemistry on LightCycler 480 system (Roche Applied Sciences). PCR primers were designed using the LightCycler Probe Design software 2.0 and table of primers is in supplementary data (S1_Table). Expression levels were determined with the

(18) Isolation and characterization of a human cementocyte-like cell line, HCY-23Brazilian Oral ResearchAugust 15, 2019Amanda Bandeira de ALMEIDA, Elis Janaína Lira dos SANTOS, Gabriel Flores ABUNA, Cristiane Salmon RIBEIRO, Márcio Zaffalon CASATI, Karina Gonzales Silvério RUIZ, Francisco Humberto NOCITI JUNIORCo., Mannheim, Germany), following the recommended protocol. Primer sequences were obtained with the LightCycler ® Probe Design Software 2.0 (Roche Diagnostics GmbH, Germany) and are listed in Table 2. Quantitative real-time PCR (qPCR) reactions

(19) Transcriptomopathies of pre- and post-symptomatic frontotemporal dementia-like mice with TDP-43 depletion in forebrain neuronsActa neuropathologica communicationsMarch 29, 2019Wu LS, Cheng WC, Chen CY, Wu MC, Wang YC, Tseng YH, Chuang TJ, Shen CJ.was performed using Roche qPCR FastMix (Roche). Primers were designed using a primer design software (LightCycler Probe Design Software 2.0 from Roche). The expression levels were normalized to gapdh, and data are represented as fold change

(20) Myocyte enhancer factor-2 and p300 interact to regulate the expression of homeostatic regulator Pumilio in DrosophilaEuropean Journal of NeuroscienceFebruary 21, 2019Wei‐Hsiang Lin, Richard A. Bainesextracted using the RNeasy microkit (QIAGEN, Hilden, Germany). PCR primers were designed with the aid of LightCycler Probe Design Software 2.0 (v1.0; Roche). Primer sequences (5’ to 3’) used were: actin‐5C (CG4027), CTTCTACAATGAGCTGCGT and GAGAGCACAGCCTGGAT;

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LightCycler^® Probe Design Software 2.0 from Roche

Description

Specifications/Features

Roche

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LightCycler^® Probe Design Software 2.0 from Roche

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